Three Passions free essay sample

Living in a Foreign Country Living away from home your country can be a really interesting and unforgettable experience, but at the same time it has very important effects on our life. I moved to U. S in 2005 and that was a hard decision to leave my country. I was only 18 years old and I had no life experience. Then, everything changed, for the best I think. One effect is that once I started a regular life away from home, I miss everything. This fact does not mean that I am unhappy but that I am aware of being on my own. Missing my family and the attention that they all paid to me is very usual detail. Details like sitting on Sunday morning watching TV with my brother or having a nice chat with my mom makes me realize how valuable family really is. I miss all the facilities that I used to have back home, like my house, my bed and my bathroom. We will write a custom essay sample on Three Passions or any similar topic specifically for you Do Not WasteYour Time HIRE WRITER Only 13.90 / page Other effect is learning how to accept a different type of society and culture in my daily life. This is a country with different customs and traditions from mine. I had to make new friends, learned other points of views, and accepted people opinion and value. The language was the most difficult to get used to it and to learn. Instead listen my native language all the time; I had to listen people talking in English. The most significant effect of living way from home is the independence behavior that had grows inside of me. Living on my own far from my family gives me a lot of experiences toward organizing my life. I have to go to school, clean my room, wash my clothes and pay all my bills, this is means that I have a good responsibility. Being independent and responsible helps me get through life every goal that I want to achieve. Living far from home, can be really hard at the beginning. I have to remember that all changes are difficult, but they are necessary to go through. Most important of all, it helps me appreciate everything that I have. I realize how lucky I am to be here, because only good opportunity has come since I got here. I have to make the best out of them.

McCulloch v. Maryland essays

McCulloch v. Maryland essays The case of McCulloch v. Maryland was brought to the United States Supreme Court in 1819. The decisions of Chief Justice John Marshall in this case would set precedence for all future cases involving the expansion of federal power and any impediment on federal powers by state governments. The first Bank of the United States (BUS) was created in 1791 as part of Alexander Hamilton's financial plan. It was opposed by Jefferson, who claimed that Congress was not specifically given the right to charter a bank. Hamilton argued (along the lines of the elastic clause) that Congress had the power to do anything not specifically forbidden in the Constitution. President Washington agreed with Hamilton and the bank was given twenty-year charter (expiring in 1811). After the war of 1812, the Madison administration sought new national development. This proved difficult with the nations finances loosely organized by state chartered banks. Inflation, and a lack of regulated currency from the state banks, prompted Madison to sign a bill creating a second BUS in 1816. The new BUS imposed strict regulation of currency on the state banks and would not accept notes from banks that had overextended themselves. In retaliation, the Maryland legislature attempted to tax the Baltimo re branch of the BUS. James McCulloch, the treasurer for this branch, refused to pay the tax and was brought to trial in the Supreme Court by the state of Maryland. Chief Justice Marshall asked two questions in this case. The first question is, "has Congress power to incorporate a bank?" Marshall states, "This government is acknowledged by all to be one of enumerated powers." Hamiltons argument Washington to charter the first BUS maintains true for this second BUS. Under the powers of the elastic clause, and simply because the Constitution does not forbid Congress from chartering a bank, it would seem acceptable for the seco ...

Marketing plan Assignment Example | Topics and Well Written Essays - 1000 words - 1

Marketing plan - Assignment Example his has been significant in enabling the business to produce high quality products at low costs, an aspect that made it possible for the business to retain its competitive advantage in the market. The goals and objectives are in line with the firm’s mission that is to bring the best computing knowledge to students, professionals, and consumers across the world, through being innovative in a way that builds its hardware, software, and internet ads. With the increasing level of competition in the market, the company goals acts as a guide in ensuring that it satisfies the tastes and preferences of the target market. This is through ensuring that it incorporates the interests of the target market in the production process. Apple Inc is active in developing new products. This is to satisfy the varying interests of the customers. Different product lines have been developed with the aim of reducing the risks and uncertainties associated with concentrating on a single product. The company has also differentiated its products. Through innovation and branding, the company’s products are unique compared to those of the competitors. This aspect has been significant in ensuring that customers remain loyal to the products despite the market being flooded with different substitutes. It has also developed a strong brand name. This is through intensive advertising in the traditional and modern media. The company has been able to create awareness on the existence of its products and services in the market. This has been achieved through personal selling, sales promotion, public relations, direct marketing, and advertising (Hassan 12). Furthermore, the company has intensified its use of social media in order to understand different aspects that affect the consumer behaviors of the target market. This has played a significant role in enabling the firm to reach a large market at very low costs. The company uses skimming and premium pricing strategies in order to penetrate new

Human security thoery and it,s implications Essay

Human security thoery and it,s implications - Essay Example The difference from traditional security is that the causes of conflict, such as economic inequality, are addressed. Achievements of those countries who adhere to the principles of human security, which include Canada, have succeeded in a number of practical ways, with the ban on anti-personnel mines and the new International Court as examples. There is a growing movement which seeks to suggests that as the United Nations stated in 1994, "the concept of security has for too long been interpreted narrowly" as the protection from physical aggression for individual countries, and thus the protection of their borders. In the global sense, the threat from possibilities such as nuclear war has been regarded as security. Human security is a massive and broad concept: a threat to it can be regarded as "any kind of unexpected or irregular discomfort". Some more concrete definition is needed. The United Nations has defined human security in the following manner, It is made up of seven specific elements, including economic, food, health, environmental, personal, community and political security. Individual governments choose to concentrate on particular elements of the security that are most suitable, or some would suggest politically expedi

Assess the contribution of Buffalo Bill to the making of the mythic Essay

Assess the contribution of Buffalo Bill to the making of the mythic West - Essay Example The myths are now history, thus transforming the nature of a time and place through the use of celebrity and propaganda. Modern culture is now built on the myths that are created through textual histories that are exaggerated and changed to support the needs of the immediate culture in creating heroes, villains, and a story to fill the spaces within the history of mankind. Early Life William Frederick Cody was born on February 26 1846 to Mary Ann and Isaac Cody in the county of Scott in Iowa. Mary Ann and Isaac Cody had traveled to Iowa as pioneers, part of the group of people who were expanding the territories in North America towards the West. In his autobiography, Cody (1978, p. 17) writes that he was the fourth child of eight children in the Cody family. When he was born, he and his family lived on a farm that they had given a Native American name, Napsinekee Place, but when he was around the age of seven he was moved to the small town of LeClair, Iowa where he had an idyllic chi ldhood. Cody (1978, p. 28) describes his childhood as an adventure, one where he stole apples from the neighbors orchard with the vigilance of the guard dog always his nemesis. He swam in the Mississippi River and took boats out on the water, although the boats were also not always his to take. He reports the story of getting stuck out in the middle of the river, he and a childhood friend having lost the oars, only to be discovered from having stolen the boat from the dock. His descriptions of the events of the his early life describe him as a child who sought adventure and wanted to experience everything, all of the thrills that would come from challenging his boundaries and the elements of his natural world. After his family made a brief and failed attempt to move to California, they moved to Walnut Grove Farm where Cody learned to trap and hunt. He became good with horses, but one event stuck out in his childhood that brought him great sorrow and involved a horse accident. His ol der brother, Samuel, rode out with Cody into town and decided to take a mare that he had been warned not to ride. Samuel, with Cody in his company on another horse, took the mare to the school where he decided to show off, but the horse reared up and then fell upon him, giving Samuel fatal injuries that took his life the next day (Cody 1978, p. 20). Some elements of this event may have contributed to the nature within Cody that led him to his celebrity. Cody’s father was involved in politics, giving him a public persona (Cody 1978, p. 19). So Cody was not unfamiliar with the concept of being known. Samuel had been a popular young boy, his gregarious personality leading the community to give him a great deal of adoration. When he died, the community felt the pain of his passing, thus even in his grief, it is possible that William saw that the effects of celebrity was the appreciation and emotional connection of a large number of people. That Cody sought fame may be traced to t his event as he recorded it in his autobiography as being transformative in his life. The second event in Cody’s life where fame and death were connected came with the stabbing and eventual death of his father. Cody’s family was moved from the farm in Iowa to Kansas, a state that was heavily involved in a debate whether to allow slavery within its borders. His father, involved in polit

Global Terrorism Index (GTI) Summary and Analysis

Global Terrorism Index (GTI) Summary and Analysis Global Terrorism The Global Terrorism Index (GTI) defines terrorism as â€Å"the threatened or actual use of illegal force and violence by a non-state actor to attain a political, economic, religious, or social goal through fear, coercion, or intimidation.† Countries are ranked on a scale out 10 based on their risk and impact of terrorism. The top five countries for the highest impact of terrorism is Iraq with a GTI score of 10/10, Afghanistan with a GTI score of 9.39/10, Pakistan with a GTI score of 9.37/10, Nigeria with a score of 8.58/10 and Syria with a GTI score of 8.12/10. Although, there are several factors that have contributed to the global pattern, three main factors include; country development, relgious idealogy and their expansion tendancies, and politcal uppheaveal. These factors have caused significant social and economic impacts for countries and their population. The map above shows the risks of terrorism globally, with red representing a severe risk graduating down to green representing a negligible risk of terrorism. As can be observed, there is a concentration of countries in Africa and areas of the Middle East such as Pakistan, Iraq, Libya, and Nigeria, which are all, developing nations or less economically developed countries (LEDC). Seventy percent of the countries in South America have a medium or high risk of terrorism. However, the global pattern of countries suffering from a medium or high risk of terrorism is scattered around the globe and includes such other countries as Brazil, Ethiopia, Iran, and even Russia. With the exceptions of some countries such as Ghana, there are clusters countries that share a common risk rating implying that neighboring countries influence each other such as the cluster in Europe, South America and Africa, where the countries within the clusters share a common risk rating. The southern hemisphere does not seem to demonstrate any particular pattern, countries with a low or a negligible risk of terrorism is randomly scattered with developed countries such as Australia and New Zealand having a negligible risk of terrorism and developing countries such as Zambia and Uruguay also having a negligible or low risk of terrorism. However, the holistic, global pattern of countries with a negligible or low risk of terrorist attacks is scattered between more economically developed countries (MEDC’S) with a cluster in Europe. Factor 1: One of the main factors that have influenced the global pattern of terrorism is country development. The global pattern of countries that have low risks and negligible risks of terrorism is in MEDC’s. This is because MEDC’s usually have a stable political system, low levels of corruption, and a high HDI (The Human Development Index) score that is a holistic indicator to give an insight on how developed a country is. For example, Norway has the highest HDI ranking with a score of 0.944; alternatively, Niger has a HDI score of only 0.337 meaning that it has the lowest level of human development in the world coupled with a severe risk of terrorism, thus showing that there appears to be a strong correlation between the level of development of a country and the risks of terrorism it faces. This is because LEDC’s suffer from lack of judicial resources and capital to be able to control violence and are more susceptible to corruption, which leads an unstable governmental system. Therefore, the higher the HDI rating the increased probability of terrorist attacks occurring in that particular country. The pie chart to the left clearly exhibits that where there is a lack of judicial control present in provides greater opportunity for terrorist organizations to carry out their activities. Syria is an example as the nation is experiencing internal and external conflict and as a consequence, terrorist attacks are more likely to occur. It is therefore seen that there seems to be a strong correlation between the pattern of global terrorism and country development, LEDC’s tending to have higher risks of terrorism. Factor 2 Another compelling factor of developing the pattern of global terrorism is extremist religious ideologies, where particular fanatics believe that they have doctrine authority to unilaterally impose their ideology on others. Demonstrating the contagious expansion of these terrorist groups into neighboring clustered nations. Extremist groups have expanded their organizations by utilizing modern day technologies such as social media and terrorist forums as a platform to advocate their beliefs and expand their territory. For example, the Islamic State of Iraq and Syria (ISIS) uses the Internet not only to spread its radical beliefs but also to recruit empower and motivate recruits from within their own homes to join their cause. The use of the Internet is a modern phenomenon used to recruit impressionable and naive youth from the west to join their campaign and to commit acts of terror in their homeland. So far, there is an estimated 12000 westerners have left their lives to expand terro r groups such as ISIS in Iraq and Syria. This affects the global pattern of terrorism because the areas in which these radical groups operate expands as they gain more power and therefore form clusters like the ones in Africa and in the middle east where terrorism crosses borders. The graph to the left (figure 12) clearly shows there are a higher proportion of religiously motivated terrorist incidents when compared to other factors over time. In 2000 the number of recorded religious incidents was 250 but in 2013 it dramatically increased to over 1500 incidents. In correlation, the graph also to the lefts (figure 12) shows the total number of terrorist incidents it is observed that as the number of global total incidents increases from 2000 so did the number of religiously motivated incidents, therefore, suggesting that the increased radicalized religious motives contributes to the increasing trend. Since the number of religiously driven attacks and the expansion of terrorist organizations has increased it indicates that the clustered countries (such as the cluster in the middle east) with a common risk rating implies that religious ideologies have caused this pattern due to porous borders. Factor 3: The last significant factor that has instigated the global pattern of terrorism is political upheavals, where governmental agendas are in conflict with groups within the society creating internal conflict. For example, in Nigeria the active extremist group Boko Haram aims to eradicate the irreligious Nigerian government to achieve radical Wahhabi interpretations of Sharia law in the country. The groups’ extreme Sunni Islamist ideology has resulted in the use of terror to enforce and proclaim their views by bombings and shootings on citizens and the government. This political upheaval due to differences in interpretations of how the government operates has caused conflict within the country and terrorist attacks on the population. Systematic political differences are what have caused the global pattern of because countries that do not have a stable and fair political system tend to be LEDC’s and suffer from high risks of terrorism, where alternatively MEDC’s like the cluster of countries in Europe which have a low or negligible risk of terrorism, tend to have a history of strong democracy and a stable government. The graph below (figure 13) shows the trends of terrorist ideologies. Political and nationalist separatist motives are more common in South America, Russia and Central America and the Caribbean. This explains the cluster of countries in South America which almost all countries have a medium or high risk of terrorism as the continent deals with corrupt governments and inequality between classes as lower socio-economic groups suffer from poverty and therefore extremist groups have formed as result. An example of this is the PCC, (Primeiro Comando da Capital ), an extremist group in South America who in May 2006 created chaos in Sao Paulo by destroying busses, construction and public buildings as well injuring civilians and police officers. This attack was driven by political and social agendas demanding improvement of Brazilian prison s. Significance Global terrorism is significant to people all over the world because of the economic and social consequences that comes as a result of it. The social impact of terrorism has mainly negative affects on people; however, there are some positive outcomes. The perception of terrorist groups depends on where the view originates. It has been said that one person’s terrorist group is another person’s ‘freedom fighter.’ Terrorism affects different people socially in different parts of the world, for example, the ongoing conflict between Russia and Chechnya; the Chechnya’n rebels are viewed as terrorists in Russia but in Chechnya they are viewed as freedom fighters. One recent example of a positive social effect of a terrorist attack is the act of bringing a community together was after ‘Sydney Siege’ in 2014. There was a campaign after the siege where people posted via Twitter with the hash tag â€Å"I’ll ride with you† offering to accompany Muslim women who were wearing the hijab on public transport to prevent harassment. Interestingly in the topic of uniting communities, terrorism has galvanized world leaders to combat collecting using combined resources to respond and aim to eradicate terrorism, for example the United nations brings developing and developed countries together to work in unity to try correct the global issue of terrorism. This creates positive long term affects for future generations as a global community is established where interdependence exists between nations. The hallmarks of terrorism are the taking of innocent lives. Approximately 150,000 people have died as a direct result of terrorist attacks over the past eight years, that is an average 43 people a day! These staggering numbers show how many families have been destroyed due to effect of radical, terrorist attacks. There are still 1,113 unidentified people from the September 11 attacks in the United States fourteen years ago; meani ng the attacks have a long-term effect for many families as they do not have closure of their loss. Another negative social affect is the mass generation of refugees. People flee out of their own countries to live in another safer country, for example, three million people have fled Syria and five million afghan refugees live in Iran as their country of origin high levels of terrorism conflict. The lasting effect has generation effects, language, financial, loss of careers health. The economic significance for people of as a result of terrorism affects every country having prolonged financial costs. Decreased tourism is a resultant effect as people do not want to travel with their families to what they perceive as risky and dangerous places so people will avoid travelling to countries with very high risks of terrorist attacks. Decrease in trade is also another cost because in the aftermath of an attack and increased prices of valuable commodities, for example, after there was an attack on 22 foreigners Saudi Arabia in 2004, the day after the incident crude oil prices rose by USD$2.50 as traders feared a US military strike in the middle east. Countries that suffer from high risks of terrorism have developing economies or unstable circumstances have drastically low investors. As security premium are higher the cost of economic involvement is high deterring foreign investors, potentially leading nations to bankruptcy. A further example of how terrorist attacks ha ve affected an economy is the aftermath of the September 11 where a range of estimates from USD$35 billion to USD$108 billion, of which the clean up cost alone of $1.3 billion; direct job losses amounted to 83,000 and further with $17 billion lost in wages in the proceeding months. One economic aspect can be noted as a positive effect of terrorism, which are the increased job opportunities in military. With escalated threats of terrorist attacks, development of the military increases thus generating more jobs for people. Also with the massive expenditure and development of military arms advancing a lot of economies especially the United States. Israel is an example of how prolonged low intensity terrorism has affected the Israeli people and the economy. Studies show that terrorism in Israel have caused a depression of economic development as in the volatile and unstable environment consumers have become cynical, corporations invest less decreasing supply and the government spends largely on defense and security measures instead of education and healthcare. Although there are some positive social and economic affects on people, the negative consequences exponentially outweigh the positives, long term impacts that terrorism has on people is catastrophic where the main consequence is loss of life which is an unfathomable tragedy that is experienced globally by all nations regardless if they are developing and developed countries.

Children in the Third World Essay -- World Poverty Essays

Children in the Third World We live in an imperfect world where poverty is a reality. Forty thousand children die per year of starvation. Over 1 billion cities face unemployment and poverty day to day. Three-fourth's of the world's largest poverty population live in the Third World Countries, which includes underdeveloped countries, mainly Africa, Asia, and Latin America. Originally the term "Third World" meant all those not supporting communism and the Western countries. Now, it is a term used to describe the poor countries of the world. These countries face hunger, diseases, illiteracy, and poor health care on a day to day basis. They have high population increases, the death rates have lowered over the years, but the birth rates are still high. Because of these increases in population and the low amount of resources available to accommodate these people, hunger is a rapidly increasing problem for the Third World. "The average spending per capita is $660 in a developing country, $13,100 in a developed country, and $19,800 in the United States." "60% of the Third World lives in extreme poverty." "I knew that poverty exists, but it never occurred to me that most of the people in this world live in poverty." (Junior Padma Krishnan) That is the problem with people today, many do not realize what a reality poverty is. That is the key word, "Reality." Many sit back and watch the commercials on children starving in Kenya or the bloated stomachs of the malnourished or undernourished in Somalia, but how many actually think about what a problem this is? What is being done to help these people? Is it fair for those in America who make millions of dollars a year to ignore this and allow these children to starve? Children by far suff... ...s imported from the poor countries, and we buy more food from 36 of the 40 countries. Is this something that we , as Americans, can be proud of? The horrific conditions experienced by the people of the third world are conditions that we can only begin to try to understand. Twenty five percent of children will die before their fifth birthday, and those who live will live without many things they need and are entitled to. Children are the future," they need to be tomorrow's solutions, not tomorrow's problems. There are many people out there working to eliminate poverty and improve living conditions for those in the third world countries, but they cannot do it alone. Everyone should take it upon themselves to do something. It may seem like an impossible to solve, but if everyone would work together, poverty would be lost and the third world would be a better place. Children in the Third World Essay -- World Poverty Essays Children in the Third World We live in an imperfect world where poverty is a reality. Forty thousand children die per year of starvation. Over 1 billion cities face unemployment and poverty day to day. Three-fourth's of the world's largest poverty population live in the Third World Countries, which includes underdeveloped countries, mainly Africa, Asia, and Latin America. Originally the term "Third World" meant all those not supporting communism and the Western countries. Now, it is a term used to describe the poor countries of the world. These countries face hunger, diseases, illiteracy, and poor health care on a day to day basis. They have high population increases, the death rates have lowered over the years, but the birth rates are still high. Because of these increases in population and the low amount of resources available to accommodate these people, hunger is a rapidly increasing problem for the Third World. "The average spending per capita is $660 in a developing country, $13,100 in a developed country, and $19,800 in the United States." "60% of the Third World lives in extreme poverty." "I knew that poverty exists, but it never occurred to me that most of the people in this world live in poverty." (Junior Padma Krishnan) That is the problem with people today, many do not realize what a reality poverty is. That is the key word, "Reality." Many sit back and watch the commercials on children starving in Kenya or the bloated stomachs of the malnourished or undernourished in Somalia, but how many actually think about what a problem this is? What is being done to help these people? Is it fair for those in America who make millions of dollars a year to ignore this and allow these children to starve? Children by far suff... ...s imported from the poor countries, and we buy more food from 36 of the 40 countries. Is this something that we , as Americans, can be proud of? The horrific conditions experienced by the people of the third world are conditions that we can only begin to try to understand. Twenty five percent of children will die before their fifth birthday, and those who live will live without many things they need and are entitled to. Children are the future," they need to be tomorrow's solutions, not tomorrow's problems. There are many people out there working to eliminate poverty and improve living conditions for those in the third world countries, but they cannot do it alone. Everyone should take it upon themselves to do something. It may seem like an impossible to solve, but if everyone would work together, poverty would be lost and the third world would be a better place.

Protein Characterization by Electrophoresis

EXPERIMENT NO. 15 PROTEIN CHARACTERIZATION BY ELECTROPHORESIS Abstract The molecular weights of protein extracts were assessed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Two sets of four protein samples, standard bovine serum albumin (BSA), invertase, egg albumin, and casein, were prepared; one set containing ? -mercaptoethanol (BME) while the other did not. These were then analyzed through SDS-PAGE with 12. 5% resolving gel, prepared using 2 M Tris-HCl at pH 8. 8 and stacking gel, prepared using 0. 0625 M Tris-HCl at pH 6. . Results showed multiple bands located on the upper half of the gel, which suggested heterogeneity of the mixture and that the samples were heavy molecules. Introduction Proteins are biological macromolecules composed of one or more polypeptides, which are polymers of amino acids. Structurally diverse, these molecules also serve a myriad of functions from enzymes, which are the biological catalysts of many physiological reactions, to components that maintain the structural integrity and organization of cells (Pratt and Cornelly, 2011). Because of this, it has been a constant effort among chemists to extract and isolate proteins to determine the mechanisms by which they act and produce the results of their reactions. Further knowledge of their biological action could translate into the discovery of many resources that could facilitate humans’ and other species’ daily lives. Electrophoresis is an analytical tool through which one can examine the movement of charged molecules in an electric field. Many modern electrophoretic techniques use a polymerized gel-like matrix as a support medium. The molecules’ migration is dependent on the applied electric field, the rigid, mazelike matrix of the gel support, and their size, shape, charge, and chemical composition. The movement of a charged molecule in an electric field is given by: v=Eq? f (1) where v is the velocity of the molecule, E is the electric field magnitude, q is the net charge of the molecule, and f is a frictional coefficient dependent on mass and shape of the molecule. Hence, it is observed that under a constant electric field magnitude, the movement is dependent on the charge-to-mass ratio of the molecule. Since each molecule is expected to have unique charges and sizes, their mobility under the electric field would also be different. Gels used in electrophoresis with different pore size may be produced by using different concentrations of cross-linking agents. Polyacrylamide gel electrophoresis (PAGE) allows enhanced resolution of sample components due to separation based on molecular sieving and electrophoretic mobility. Because of the presence of a continuous network of pores in the gel, large molecules do not move easily through the medium compared to smaller ones. Two types of gels are used: the resolving and stacking gels, each having different concentrations of acrylamide and of different pH and ionic strengths. The denaturants sodium dodecyl sulfate (SDS), a detergent, and ? -mercaptoethanol (BME), a reducing agent, are frequently used in PAGE. The action of these two denaturating agents cause the production of polypeptide chains of constant charge-to-mass ratios and uniform shapes due to the SDS molecules binding with the hydrophobic regions of the denatured polypeptide and masking the native charge of the protein by its negative charge. This restriction, coupled with the fact that mobility of the SDS-protein complexes are based on molecular size, forms the basis of the electrophoretic determination of purity and molecular weight (Boyer, 1993). This experiment will utilize SDS-PAGE to assess the molecular weights of the extracted proteins invertase, albumin, and casein, along with standard bovine serum albumin. The effect of the presence of ? -mercaptoethanol was also investigated. Methodology With the glass plates clean, the gel apparatus was first set up with the comb inserted between the glass plates. It was made sure of that the set-up would not leak by allowing a little amount of distilled water to enter it, which was discarded afterwards. A mark, one centimeter below the teeth of the comb, was placed on the glass plate. The resolving gel, at 12. 5% gel, was then prepared in an Erlenmeyer flask. Using a micropipette, 1450 ? L of 40% stock acrylamide, 775 ? L of 2% stock bisacrylamide, 875 ? L of 2 M Tris-HCl at pH 8. 8, and 1500 ? L of distilled water were measured and mixed in the Erlenmeyer flask. Afterwards, 47 ? L of 10% sodium dodecyl sulfate (SDS) and 40 ? L of freshly prepared 10% ammonium persulfate (APS) were added to the mixture. Then, ten microliters (10 ? L) of tetramethylethylenediamine (TEMED) was added and, after mixing it by swirling not more than three times, the mixture was poured into the gel apparatus with the aid of a micropipette up to the mark. The gel was then overlaid with a small amount of isobutanol-water mixture before it would start to harden. After the gel has completely polymerized, the isobutanol mixture was removed from the apparatus. Two pairs of two resolving gels were prepared as one pair would be used for samples containing ? mercaptoethanol and another pair for those that do not contain the said chemical. The stacking gel was prepared by taking 265 ? L of 40% stock acrylamide, 140 ? L of 2% stock bisacrylamide, 350 ? L of 0. 625 M Tris-HCl at pH 6. 8, and 940 ? L of distilled water and mixing all four in an Erlenmeyer flask. Afterwards, 25 ? L of 10% SDS and 60 ? L of 10% APS were added to the one in the flask. Immediately before the solution was added, 5 ? L of TEMED was added to it and swirled not more than three times, similar to that done with the resolving gel. This mixture was then rapidly transferred by a micropipette over the resolving gel and, after placing the comb over it, left to harden. The samples were prepared by getting 100 ? L of the protein sample, 20 ? L of distilled water, and 80 ? L of loading buffer with ? -mercaptoethanol in plastic tubes for the electrophoresis of the samples containing ? -mercaptoethanol. For those samples not containing the latter reagent, 80 ? L of the loading buffer was added. The same procedure was done for 100 ? L of bovine serum albumin. These were then placed in a boiling water bath for 10 minutes after which these were immediately immersed in an ice water bath for 3 minutes. The protein samples used were invertase, albumin, and casein. The loading buffer was prepared by mixing 2. 5 mL of 10% SDS, 2. 5 mL of 0. 625 M Tris-HCl at pH 6. 8, 2. 5 mL of 10% glycerol, and 5. 0 g of 0. 02% bromophenol blue, and diluting to 25 mL with distilled deionized water. Eight tubes were done all-in-all. The gel slabs were then placed in the gel chamber. The gel chambers were then filled with gel running buffer, making sure that the gel was completely immersed. This buffer was prepared by mixing 3. 0 g Tris base, 14. 4 g glycine and 1. 0 g SDS, and diluting to 1 L with distilled deionized water.. The set-up was then placed on a level surface. At this point, the comb was removed in one fluid motion to ensure that the wells would have straight edges. Ten microliters (10 ? L) of the samples with ? -mercaptoethanol was loaded into the wells using a micropipette. With the voltage set at 100 V and the protective electrode covering placed over the set-up, the gel was run until the dye reaches a level of 1 cm above the bottom of the gel slab. This was done again for those samples without the ? -mercaptoethanol. After the gels have been run, the gel slabs were transferred from the glass plates to a flat-bottom container where a small amount of staining solution was added until the slabs were completely immersed. This solution was prepared by mixing 50 mL of methanol, 10 mL of glacial acetic acid, and 0. 25 mg of Coomassie Brilliant Blue R250, and diluting to 100 mL with distilled deionized water. After that, the background staining was removed by several washings of destaining solution. This solution was prepared by mixing 25 mL of 95% ethanol and 5 mL of glacial acetic acid, and diluting to 100 mL with distilled deionized water. Results and Discussion Polyacrylamide Gel Electrophoresis (PAGE) served as an effective tool in the characterization of protein standards and extracts because of the gel’s high resolving power for molecules up to 106 Da, accommodation of larger sized samples, an inert enough matrix with respect to the migrating entities, and physical stability of the matrix (Boyer, 1993). Polyacrylamide gels were prepared by the catalyzed and cross-linked polymerization of the acrylamide-bisacrylamide mixture. The polymerization reaction was facilitated by ammonium persulfate (APS), the polymerizing agent, due to its inherent instability and, hence, its tendency to decay and to give rise to molecules initiating these polymerization. Tetramethylethylenediamine (TEMED) was introduced to catalyze the decay of APS. Figure 1 presents the general equation for the polymerization reaction of the acrylamide-bisacrylamide mixture (Encor Biotechnology, Inc. , 2011). Figure 1. The polymerization reaction of the Acrylamide-bisacrylamide in the presence of ammonium persulfate and TEMED as the polymerizing agent and the catalyst respectively (Thermo Scientific, Inc. , 2011) Polymerization proceeded with the opening of an acrylamide double bond, allowing it to react with another acrylamide to produce a linear polyacrylamide. Cross links were generated through the incorporation of bisacrylamide into the linear polyacrylamides. Since molecular oxygen would react with the free radical sulfate ions (SO42-) thereby inhibiting polymerization, degassing was necessary. Furthermore, the tendency of molecular oxygen to react with SO42- would also be the reason why it would be necessary for PAGE gels to be poured into tubes or between glass plates instead of horizontal apparatuses. However, the degassing step was not done due to the unavailability of a degassing chamber. Isobutanol was added on top of the gel to also prevent the entry and accumulation of O2 (Encor Biotechnology, Inc. , 2011). Gel pore size is inversely proportional to the concentration of acrylamide. Therefore, to generate a broad and efficient range of protein separation, a discontinuous gel system was formulated, having a low acrylamide content on top and a high acrylamide content at the bottom. The capability of Tris-HCl to facilitate the propagation of electric current through the matrix qualified it as an appropriate loading buffer. It allowed the proteins to be drawn by the current through the sieving matrix slab (Thermo Scientific, Inc. , 2011). The polyacrylamide gel electrophoreses set-up had three important features. First, a stacking gel was cast over a resolving gel. Second, the two gel layers had different ionic strengths and pH. Third, the stacking gel had a lower acrylamide concentration and a lower pH. These conditions allowed the protein molecules to first concentrate into a tight band before entering the resolving solution. In this experiment in particular, the charge of the protein was kept uniform all throughout using sodium dodecyl sulfate (SDS), a powerful detergent that would denature the protein and would leave it evenly negatively charged. Also, ? -mercaptoethanol was added to cleave the disulfide bonds, enforcing completely disrupted secondary, tertiary, and quarternary structures. Prior to the loading of the sample, the discontinuous gel system was immersed in a glycine-Tris buffer prepared at pH 8. 8. At this pH, the two form of glycine – its Zwitterion ion and glycinate – would exist in equilibrium. H3N+CH2COO- – H2NCH2COO- + H+(2) When the voltage was turned on, the entry of buffer ions (glycinate and H+) to the stacking gel (pH 6. 8) shifted the equilibrium to the left, increasing the concentration of glycine’s Zwitterion ion, which would have a zero net charge, and therefore, would be electrophoretically immobile. Since the protein molecules would still be anionic at pH 6. , they would replace the nonmobile glycine molecules in order to keep the current running. As such, the relative mobilities of the ions in the stacking gel would be Tris base > protein sample > glycinate. Furthermore, the thin band observed in the upper gel would actually pertain to the protein molecules sandwiched between the Tris-base and the glycinate ions . The resolving gel, on the other hand, had a pH of 8. 8. When the ionic front reached it, the equilibrium of glycine species shifted to the right. The increase in pH and decrease in pore size retarded the movement of proteins and rendered the glycinate ions greater mobility. The relative rates of movement then became Tris-base > glycinate ions > protein samples. From there, it was the mass of the protein molecules that governed their mobility and thus identified them (Boyer, 1993). For qualitative analysis of results, the Coomassie brilliant blue dye (R-250), being the most popular staining reagent for the electrophoresis of protein samples, was used. Its mechanism of binding to the basic and hydrophobic groups of proteins manifested in the dull, reddish-brown to intense blue color change of the solutions. The staining method was started with the water wash of the gel cast to remove the electrophoresis buffers from the matrix. The matrix was then washed with methanol followed by glacial acetic acid to prevent the diffusion of protein bands form the matrix. The treatment with the dye followed. Lastly, destaining measures were employed to get rid of excess dye from the background gel matrix. This would allow a clear visualization of the bands that had formed (Thermo Scientific, Inc. , 2011). Figures 2 and 3 are photographs of the two gels after incubation and subjection to the dye. Figure 2. Photograph of 1st gel Figure 3. Photograph of 2nd gel In figure 2, multiple bands existed. This could suggest that the samples had other components. These could come in the form of other proteins, contaminants, or other impurities. Nonetheless, any of these possibilities suggest one thing; the sample is not pure although there are occasional times when homogeneous samples result to multiple bands due to degradation during the electrophoresis procedure (Boyer, 1993). Also, the identity of the proteins could have been determined if there were standards or â€Å"markings† to compare these bands with. However, there were none. The only information that could be extracted from the photographs could be that the proteins in the samples were heavy that they were only located on the upper half of the gel. Conclusion The separation of biomolecules according to charge, size, and shape through electrophoresis could give significant information such as the purity, molecular weight, and, hence, the identity of the biomolecule. In this experiment, the multiple bands produced in the gel set-ups suggested that the samples were heterogeneous. Their location in the gel suggested that the proteins were relatively heavy ones. To gain more valuable information rom these data, it is recommended that a set of standard solutions be also run on the gel so that they could be used as references for the identification of the proteins in the samples. Also, the protein’s exact molecular size could be determined by preparing a calibration curve from a set of standard solutions of proteins, with of course, known concentration. The curve should be a plot of the logarithm of the molecular weight of the protein versus its mobility in the gel matrix. From this curve, the molecular weight of the protein in the sample solutions could be extrapolated. References 1. Boyer, Rodney. Modern Experimental Biochemistry. Third Edition. San Francisco, USA: Benjamin/Cummings, 1993. Scribd. Web. 29 November 2011. 2. Encor Biotechnology, Inc. â€Å"SDS-Polyacrylamide Gel Electrophoresis (SDS-PAGE). † Encor Biotechnology, Inc. Protocols. Encor Biotechnology, Inc. , 2011. Web. 30 November 2011 < http://www. encorbio. com/protocols/SDS-PAGE. htm>. 3. Thermo Scientific, Inc. â€Å"SDS-Polyacrylamide Gel Electrophoresis (SDS-PAGE). † Thermo Scientific, Inc. Protein Methods Library. Thermo Scientific, Inc. , 2011. Web. 30 November 2011 < http://www. piercenet. com/browse. cfm? fldID=21518847-2D72-475F-A5B9-B236EC5B641E >.

Why Lithium Batteries Catch Fire

Why Lithium Batteries Catch Fire Lithium batteries are compact, lightweight batteries that hold considerable charge and fare well under constant discharge-recharge conditions. The batteries are found everywhere  -   in laptop computers, cameras, cell phones, and electric cars. Although accidents are rare, those that do occur may be spectacular, resulting in an explosion or fire. In order to understand why these batteries catch fire and how to minimize the risk of an accident, it helps to understand how the batteries function. How Lithium Batteries Work A lithium battery consists of two electrodes separated by an electrolyte. Typically, the batteries  transfer electrical charge from a lithium metal cathode  through an electrolyte consisting of an organic solvent containing lithium salts over to a carbon anode. The specifics depend on the battery, but lithium-ion batteries usually contain a metal coil and a flammable lithium-ion fluid. Tiny metal fragments float in the liquid. The contents of the battery are under pressure, so if a metal fragment punctures a partition that keeps the components separate or the battery is punctured, the lithium reacts with water in the air vigorously, generating high heat and sometimes producing a fire. Why Lithium Batteries Catch Fire or Explode Lithium batteries are made to deliver high output with minimal weight. Battery components are designed to be lightweight, which translates into thin partitions between cells and a thin outer covering. The partitions or coating are fairly fragile, so they can be punctured. If the battery is damaged, a short occurs. This spark can ignite the highly reactive lithium. Another possibility is that the battery can heat to the point of thermal runaway. Here, the heat of the contents exerts pressure on the battery, potentially producing an explosion. Minimize the Risk of Lithium Battery Fire The risk of fire or explosion increases if the battery is exposed to hot conditions or the battery or internal component is compromised. You can lessen the risk of an accident in several ways: Avoid storing at high temperatures. Dont keep batteries in hot vehicles. Dont allow a blanket to cover your laptop. Dont keep your cell phone in a warm pocket.  You get the idea.Avoid keeping all your items containing lithium-ion batteries together. When you travel, especially on a plane, youll have all your electronic items in one bag. This is unavoidable because the batteries have to be in your carry-on but usually, you can keep some space between battery-containing items. Although having lithium-ion batteries in close proximity does not increase the risk of a fire, if there is an accident, the other batteries can catch fire and make the situation worse.Avoid overcharging your batteries. These batteries do not suffer memory effect as badly as other types of rechargeable batteries, so they can be discharged and recharged many times nearly back to their original charge. However, they do not fare well if they are completely drained before recharging or are over-charged. Car chargers are notorious for overcharging batteries. Using any charger other than the one intended for the battery can increase the risk of damage.

Dubliners, death and paralysis Essay Example

Dubliners, death and paralysis Essay Example Dubliners, death and paralysis Essay Dubliners, death and paralysis Essay even Mr Duffys face is brown, the brown tint of the Dublin streets. a symbol of a decaying Mr Duffy and a decaying Dublin. When Mrs Sinico and Mr Duffy start to meet up enjoying each others company reading, listening to music and talking, Mr Sinico pays no attention to the relationship forming between his wife and Mr Duffy He had dismissed his wife so sincerly from his gallery of pleasures that he did not suspect anyone else would take an interest in her. This showed the death of the Sinico marriage they were just co existing together. When Mrs Sinico advances her hand in intimacy to Duffy, but he retreats repulsed by her failure to understand, and he breaks off the relationship. When Mr Duffy is sitting in a cafe four years later and he finds the article called a painful case which is on buff coloured paper (a yellowish brown. ) Showing the death of her character, Mr Duffy then began to feel guilt and wonder did his rejection of her four years ago result in her sucide? He automatically turns it around to feel sorry for himself, when really he should be feeling sorry for Mrs Sincio or her family. Joyce as we know from the picture of the priest in Eveline, uses the colour yellow for corruption and brown for death, meaning that she has died a corrupt and weak woman, Mr Duffy also shows digust in her behaviour and her death and digust that he allowed himself to get clost to such a woman. Then he has a moment when he thinks of her in pity, pity for her loniness, however because of his selfcentred nature he starts to think of his own loniness and realises that he is not content with his hermitic lifestyle to which he has become accustomed to. We know this by his reaction to the couple in the park, who are having sex and making him feel even more alone in the world. He realises that his concern with orderand rectitude shut her out of his life, and this concern stops him from living fully, he is not interested in begining a new phrase in his life, but instead he bitterly accepts his loneliness. Mr Duffy seems incapable of genuine feeling and emotion and this seems to be inpart to blame for his painfull insularity and his obession with his predictable life costs him a golden chance at love and happiness. A painful case ended where it began with Mr Duffy alone. Corley and Lenehan refuse to grow old in Two Gallants, trapped in the paralysis that Joyce saw central to Dublin, therefore this story belongs to the adolscent stories of the Dubliners, even though the two are in their thirties. Corley and Lenehan are leeches, to give this story the title two gallants Joyce was using this title as a form of ironry because neither Corley or Lenehan are gallants. (gallants meaning to be brave or herotic. Corley takes advantage of the women, that he seduces, Lenehan does the same to friends, bartenders and Corley also. Donald Torchiana suggests that the stroy is on allegorical tale about the Anglo-Irish Ascendency and the culture or parasitiam and exploitation which they bred in Ireland. Their is evidence to support this theory, which would be in the referneces to the ascendency in the Kildare Street Club and in place names such as Shelbourne Hotel. The girl could be seen as a symbol of Ireland, representing Irelands depraved condition under colonial rule. Ireland is being prosituted by her English conquerors. This ambiguous connection between Lenehan and the harp is a national reference. When Lenehan enjoys his meal later on in the story his meal has the colours of the Irish flag the green peas and the orange ginger beer symbolishing the flag of Ireland and therefore in Joyces view Lenehan is a steorotypical Dubliner. At the start of the story Joyce describes Dublin as having a warm grey evening air and a gaily coloured crowd. Which Joyce is using colour to show the decay in Dublin the city itself and the people in it. Lenehan and Corley are both spirtualy dead in this story, they are scamming to get what they want, which in my opionion Joyce thought was quite typical of the people of Dublin using betrayel to make money, duping maids into stealing from their employers. Every main character in the three stories that I choose had deep moral and spritual paralysis and spritual death, with each character having a decision or situation which either inhibitits their well being or self estem and prohibits their ability to move forward. All three stories that I studied didnt have a happy ending, Joyce saw Dublin to never have a happy ending so he shows this in his stories. Dubliners are paralysed from acting or living decisively or even consciously. Joyce once told his brother stonislaus the city is suffering from hemiplegia of the will (Stuart Gilbert, ed. the letters of James Joyce, Faber 1957. ) He also described his intention to write The Dubliners as the desire to betray the soul of the hemiplegia or paralysis which may consider a city. The theme of praralysis and death is evident in every story in the Dubliners which I have come to the conclusion that this the way Joyce sees Dublin as a dead city diseased with paralysis, a city which one would want to escape from.

Business and Government Customer Decision Making Essay

Business and Government Customer Decision Making - Essay Example Often, straight rebuy is a purchase from an "approved list" of suppliers which satisfactory met the standard set buy the buyer. In order for the suppliers to maintain its relationship with the business buyer, it maintains its product or service quality. Companies utilizing straight rebuy are often automated in order to save reordering cost and time. Globally, Dell, Inc. is recognized as an organization which pursues strategic partnership with its various suppliers. Through the use of technology, the company shares the level of its material inventory with its current suppliers. These enables it to facilitate straight rebuy once its input goes below the optimum quantity. Aldi, the international retailer is also using a straight rebuy in replenishing its grocery shelves. It should be noted that the retailer has an extensive network of supplier for its merchandise. The company employs a Point of Sale system which tells suppliers when to deliver the needed goods. Modified rebuy happens when a business organization wants to purchase a component but would like to alter product specifications, prices, delivery requirement, or other terms (Widing 2002, p.155). As opposed to straight rebuy this buyclass may consider new suppliers to provide its new needs. The company also gathers information in its quest of finding the appropriate supplier.

Marketing Mix Assignment Example | Topics and Well Written Essays - 1000 words

Marketing Mix - Assignment Example rious education programs through DVDs, online lessons, interactive museum lessons, as well as support for families, kids, and teachers on creating awareness about art in the entire USA. The theme of the arts is the traditional American and European paintings though there has been the incorporation of the modern, a perfect example being the Ginevra de Benci by Leonardo. Fundraising is done on the basis of becoming a member by donating a gift of $1,000 enabling one to enjoy exhibitions, education programs and other programs. Besides, the museum owns stationery, and other art galleries can be accessed online. The Getty is among the best art museums in Los Angeles, having a population of 3.884 million, with the cheapest collection where everything almost accessed freely (White 24). There is free admission, besides, the exhibits, gallery talks and the curator-led tours are provided at no additional fees. The museum is unique as it stays open until 9 pm on Saturdays and parking is not charged after 5p.m during the weekends. The theme of the arts focuses on modern architecture that adds to the expensive city view in addition to the labyrinth of gardens adds to the impressiveness of the museum. Besides, there is the exhibition of the Western art including the illuminated manuscripts, paintings, photos, drawings and other decorative arts that have been provided for by American and European artists. Getty centre has various shops spread across LA with the main shop situated in the Entrance Hall offering books, gifts, jewelry, and stationery. There is also the children shop in the West Pavilion and also the Centre for Photographs Shop also offering art galleries. There education programs provided by the Education department that organizes partnerships, programs, social media lessons, and resources for training. One can become a member of the museum by signing up for the mailings to receive the e-newsletters or can contribute in the form of donating arts. The Museum of